The aim of proteomics is to identify and characterize all the proteins in an organism. The major restrictions in protein identification are the large number of proteins and the huge differences in abundance. These restrictions mean low abundance, critical proteins are often masked and are therefore hard to identify and purify. One key tool in resolving this issue is to utilize protein fractionation techniques.
The Protein Fractionation kit teaches common protein fractionation techniques used during protein purification, including acid and salt fractionation. The individual or combined use of acid and salt fractionations allows for the concentration and enrichment of proteins into defined fractions dependent on their precipitation at differing salt concentrations and/or pH. Students understand the importance of enrichment of target proteins in the scheme of purification of protein molecules. This lab activity involves preparation of a crude protein extract and fractionation of proteins by incremental changes in pH and salt concentration.
Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides.
Applications
This lab activity teaches the following:
- Strategies in protein purification.
- Principles of protein fractionation.
- Protein fractionations by changing pH.
- Protein fractionation by increasing salt concentration.