- Promotes neuronal cell attachment and differentiation for a variety of applications
Corning® BioCoat™ Poly-D-Lysine/Laminin (PDL/LM) and Poly-L-Ornithine/Laminin (PLO/LM) Cellware is suitable for culturing many different types of peripheral and central nervous system (CNS) networks and is useful for promoting neural cell attachment and differentiation. Corning® BioCoat™ Laminin/Fibronectin (LM/HFN) Cellware provides an in vitro environment that promotes cell attachment and extensive process formation. In addition, this substrate upregulates receptor systems that are integral to neuronal communications. Corning® BioCoat™ LM/HFN Cellware facilitates the study of pharmacological or physiological events of the CNS by providing molecular cues required to produce neuronal cultures capable of responding to many important neurotransmitters, neuropeptides, and excitatory amino acids such as glutamate, NMDA, and kainate.
This line of tissue culture vessels have a uniform application of PDL/LM, PLO/LM, or LM/HFN. Corning® BioCoat™ PDL/LM, PLO/LM, and LM/HFN Cellware are manufactured in a highly controlled environment and rigorously tested to assure product consistency and performance.
Applications include:
- Enhancement of neuronal cell attachment to plastic and glass
- Promotion of neurite outgrowth
- Culture of glial cells as feeder layers for neurons
- Construction of neural cell model systems to study CNS function development and diseases
Quality Control:
PDL/LM and PLO/LM are tested for ability to promote neurite outgrowth with primary rat cerebellar granule cells (RCG) and NG-108 rat glioma/mouse neuroblastoma cells. Tested and found negative for bacteria and fungi.
Storage and Stability:
Cellware stable for at least three months at 2-8°C. Do not freeze. Coverslips and CultureSlides stable for at least three months from date of shipment when stored at 2-8°C.